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1x tris acetate edta tae buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x tris acetate edta tae buffer
    1x Tris Acetate Edta Tae Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/1x+tae+buffer/TAE+(10X)%2C+TRIS+%2B+acetate+%2B+EDTA/pm42247714-142-19-16
    Average 95 stars, based on 1 article reviews
    1x tris acetate edta tae buffer - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Article Title: The effect of PEGylation on surface tethering of liposomes via DNA nanotechnology
    Article Snippet: .. DNA and loading dye mixtures were added to each lane of the cast gel and run in 1x TAE buffer on the Invitrogen Mini Gel Tank Electrophoresis system (Invitrogen Inc.) connected to a Lively 300V Power Supply (Major Science Co.). .. Gels were run at 150 V over 90 min 5 μl of 10,000× concentrated SYBRTM Gold Nucleic Acid Gel Stain (Invitrogen Inc.) was added to 50 ml MilliQ with the gel submerged under constant shaking to post-stain for 4 min. Gels were imaged using InvitrogenTM iBrightTM FL1500 Imaging System (Thermo Fisher Scientific Inc).

    Agarose Gel Electrophoresis:

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Article Title: Enrichment and Reduction of Microsatellite Regions in the Myxoma Virus Genome Following Species Jump to the Iberian Hare ( Lepus granatensis )
    Article Snippet: Additional PCR amplifications using the primers described in section were carried out in a final volume of 50 μL using the LA Taq kit (Takara) following the manufacturer’s recommendations. .. Amplicons were analysed on a 1% agarose gel (Agarose D1 Low EEO; Conda Laboratories) made with 1X TAE buffer and stained with RedSafe (iNtRON Biotechnology) using the O’GeneRuler 1 kb Plus DNA Ladder marker (Thermo Scientific) as a reference. .. Gels were visualised using a Gel Logic 212 Pro Camera (Carestream and Carestream SI ME Software).

    Article Title: mRNAbow : A versatile gene expression system for multiplexed fluorescent imaging using optimized in vitro transcribed mRNA
    Article Snippet: .. Briefly, 500 ng of RNA per sample was mixed with 6x loading dye and resolved on a 1% agarose gel prepared in 1x TAE buffer containing SYBR Safe (Invitrogen). .. Gels were run at 100 V for 30-40 min, and RNA integrity was assessed by band size under UV illumination using a ChemiDoc system (Bio-Rad).

    Article Title: Microbial biodiversity in Tunisian olive grove soils: a reservoir of phytopathogenic fungi and potential beneficial microorganisms
    Article Snippet: .. The PCR products, stained with GelRed ® (Biotium Inc., Fremont, CA, USA), were checked after electrophoretic separation on 1.5% agarose gel in 1X TAE buffer under UV light by comparison with a 100 bp DNA Ladder (ThermoFisher Scientific, Waltham, Massachusetts, USA). .. Before sequencing, the PCR products were purified with an enzymatic EXO/FastAP mixture (Exonuclease I and FastAP thermosensitive alkaline phosphatase, ThermoFisher Scientific, Waltham, Massachusetts, USA).

    Staining:

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Article Title: Enrichment and Reduction of Microsatellite Regions in the Myxoma Virus Genome Following Species Jump to the Iberian Hare ( Lepus granatensis )
    Article Snippet: Additional PCR amplifications using the primers described in section were carried out in a final volume of 50 μL using the LA Taq kit (Takara) following the manufacturer’s recommendations. .. Amplicons were analysed on a 1% agarose gel (Agarose D1 Low EEO; Conda Laboratories) made with 1X TAE buffer and stained with RedSafe (iNtRON Biotechnology) using the O’GeneRuler 1 kb Plus DNA Ladder marker (Thermo Scientific) as a reference. .. Gels were visualised using a Gel Logic 212 Pro Camera (Carestream and Carestream SI ME Software).

    Article Title: Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cells.
    Article Snippet: .. Briefly, 2% agarose (VWR #490001-580, Radnor, PA, USA) gel was prepared in 1x TAE buffer (Tris-acetate-EDTA, VWR #75800-922) with SYBR Safe DNA Gel Stain (Thermo Fisher Scientific #S33102). .. Gel was loaded with GeneRuler DNA Ladder Mix (Thermo Fisher Scientific #SM0333) and CDS amplification products mixed with NEB Gel Loading Dye (New England Biolabs #B7024S, Ipswich, MA, USA) and run at 150 mA for 45 minutes.

    Article Title: Microbial biodiversity in Tunisian olive grove soils: a reservoir of phytopathogenic fungi and potential beneficial microorganisms
    Article Snippet: .. The PCR products, stained with GelRed ® (Biotium Inc., Fremont, CA, USA), were checked after electrophoretic separation on 1.5% agarose gel in 1X TAE buffer under UV light by comparison with a 100 bp DNA Ladder (ThermoFisher Scientific, Waltham, Massachusetts, USA). .. Before sequencing, the PCR products were purified with an enzymatic EXO/FastAP mixture (Exonuclease I and FastAP thermosensitive alkaline phosphatase, ThermoFisher Scientific, Waltham, Massachusetts, USA).

    SYBR Green Assay:

    Article Title: Microbial signatures define the ecosystem functions of the pelagic microbiome in a basin-scale, Southwest Atlantic Ocean.
    Article Snippet: .. DNA integrity was determined by electrophoresis on a 1% (v/v) agarose gel prepared with 1X TAE buffer (0.04 M Tris, 1 M glacial acetic acid, 50 mM EDTA, pH 8), and stained with SYBR Green (Thermo Fisher Scientific, São Paulo, Brazil). .. DNA concentration and integrity were determined using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, São Paulo, Brazil), according to the manufacturer’s instructions.

    Marker:

    Article Title: Enrichment and Reduction of Microsatellite Regions in the Myxoma Virus Genome Following Species Jump to the Iberian Hare ( Lepus granatensis )
    Article Snippet: Additional PCR amplifications using the primers described in section were carried out in a final volume of 50 μL using the LA Taq kit (Takara) following the manufacturer’s recommendations. .. Amplicons were analysed on a 1% agarose gel (Agarose D1 Low EEO; Conda Laboratories) made with 1X TAE buffer and stained with RedSafe (iNtRON Biotechnology) using the O’GeneRuler 1 kb Plus DNA Ladder marker (Thermo Scientific) as a reference. .. Gels were visualised using a Gel Logic 212 Pro Camera (Carestream and Carestream SI ME Software).

    Polymerase Chain Reaction:

    Article Title: Microbial biodiversity in Tunisian olive grove soils: a reservoir of phytopathogenic fungi and potential beneficial microorganisms
    Article Snippet: .. The PCR products, stained with GelRed ® (Biotium Inc., Fremont, CA, USA), were checked after electrophoretic separation on 1.5% agarose gel in 1X TAE buffer under UV light by comparison with a 100 bp DNA Ladder (ThermoFisher Scientific, Waltham, Massachusetts, USA). .. Before sequencing, the PCR products were purified with an enzymatic EXO/FastAP mixture (Exonuclease I and FastAP thermosensitive alkaline phosphatase, ThermoFisher Scientific, Waltham, Massachusetts, USA).

    Comparison:

    Article Title: Microbial biodiversity in Tunisian olive grove soils: a reservoir of phytopathogenic fungi and potential beneficial microorganisms
    Article Snippet: .. The PCR products, stained with GelRed ® (Biotium Inc., Fremont, CA, USA), were checked after electrophoretic separation on 1.5% agarose gel in 1X TAE buffer under UV light by comparison with a 100 bp DNA Ladder (ThermoFisher Scientific, Waltham, Massachusetts, USA). .. Before sequencing, the PCR products were purified with an enzymatic EXO/FastAP mixture (Exonuclease I and FastAP thermosensitive alkaline phosphatase, ThermoFisher Scientific, Waltham, Massachusetts, USA).



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